Telleria Jenny, Barnabé Christian, Hide M., Banuls Anne-Laure, Tibayrenc Michel. (2004). Predominant clonal evolution leads to a close parity between gene expression profiles and subspecific phylogeny in Trypanosoma cruzi. Molecular and Biochemical Parasitology, 137 (1), p. 133-141. ISSN 0166-6851.
Titre du document
Predominant clonal evolution leads to a close parity between gene expression profiles and subspecific phylogeny in Trypanosoma cruzi
Telleria Jenny, Barnabé Christian, Hide M., Banuls Anne-Laure, Tibayrenc Michel
Source
Molecular and Biochemical Parasitology, 2004,
137 (1), p. 133-141 ISSN 0166-6851
We investigated the relationships between overall phylogenetic diversity in Trypanosoma cruzi evidenced by multilocus markers (MLEE and RAPD) on the one hand, and gene expression patterns, revealed by mRNA analysis on the other hand. Nineteen laboratory-cloned stocks representative of this parasite’s overall phylogenetic diversity and ecogeographical range were analyzed using random amplified differentially expressed sequences (RADES). The bat trypanosome T. cruzi marinkellei was taken as outgroup. The profiles obtained showed that RADES polymorphism cannot be considered as a simple subsample of general RAPD polymorphism. Indeed, many RADES bands were not present in general RAPD profiles, and vice versa. Phylogenies obtained from RADES on the one hand, and MLEE/RAPD on the other hand, were very similar. This suggests that in spite of the recent observation of hybrid genotypes and mosaic genes in T. cruzi, clonal evolution in this parasite has been preponderant enough on an evolutionary scale to carve the polymorphism on all types of DNA sequences, including expressed genes, although these genes are assumed to undergo natural selection pressure contrary to noncoding sequences and neutral polymorphisms.
Plan de classement
Glossines, réduviides et trypanosomiases [052GLOTRY]
Descripteurs
TRYPANOSOMIASE HUMAINE ; MALADIE DE CHAGAS ; AGENT PATHOGENE ; GENETIQUE DE POPULATION ; PHYLOGENIE ; RECOMBINAISON GENETIQUE
