Publications des scientifiques de l'IRD

Konan J. A., Guyot Romain, Koffi K. K., Vroh-Bi I., Zoro A. I. B. (2020). Molecular confirmation of varietal status in bottle gourd (Lagenaria siceraria) using genotyping-by-sequencing. Genome, 63 (11), p. 535-545. ISSN 0831-2796.

Titre du document
Molecular confirmation of varietal status in bottle gourd (Lagenaria siceraria) using genotyping-by-sequencing
Année de publication
2020
Type de document
Article référencé dans le Web of Science WOS:000586846000003
Auteurs
Konan J. A., Guyot Romain, Koffi K. K., Vroh-Bi I., Zoro A. I. B.
Source
Genome, 2020, 63 (11), p. 535-545 ISSN 0831-2796
Bottle gourd (Lagenaria siceraria) is an important food, medicinal, and utilitarian crop with a large pan-tropical distribution. Two morphologically different types in the siceraria subspecies are sufficiently different to be considered as varieties, but they are assigned into different taxonomic ranks. Genotyping-by-sequencing (GBS) of 95 different accessions from the Nangui Abrogoua University collection was used to confirm the varietal status in bottle gourd. This analysis produced 22 575 single-nucleotide polymorphisms (SNPs). Cluster analyses conducted with 2250 (9.96%) SNPs distinctly separated hard-shelled from soft-shelled types. Analysis of 23 SNPs located in 11 genes coding for traits that differentiate the two types of gourds revealed that genes in the soft-shelled types had about 21% fewer SNPs than genes within hard-shelled gourds, but the latter had more non-synonymous SNPs. Cluster analyses conducted with the 23 SNPs fitted well with the structure defined by the 2250 SNPs, suggesting the implication of these SNPs in the varietal differentiation of bottle gourd. These nucleotide changes along with the genetic relationships between the accessions provide molecular proof supporting the status of two varieties. To prevent the confusion inherent in the use of synonyms and homonyms in bottle gourd, we suggest the terms hard-shelled and soft-shelled to designate, respectively, the varieties used as utensils and those grown for their edible seeds.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Sciences du monde végétal [076]
Description Géographique
BURKINA FASO ; COTE D'IVOIRE ; MALI
Localisation
Fonds IRD [F B010079962]
Identifiant IRD
fdi:010079962
Contact