@article{fdi:010079962,
  title = {{M}olecular confirmation of varietal status in bottle gourd ({L}agenaria siceraria) using genotyping-by-sequencing},
  author = {{K}onan, {J}. {A}. and {G}uyot, {R}omain and {K}offi, {K}. {K}. and {V}roh-{B}i, {I}. and {Z}oro, {A}. {I}. {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ottle gourd ({L}agenaria siceraria) is an important food, medicinal, and utilitarian crop with a large pan-tropical distribution. {T}wo morphologically different types in the siceraria subspecies are sufficiently different to be considered as varieties, but they are assigned into different taxonomic ranks. {G}enotyping-by-sequencing ({GBS}) of 95 different accessions from the {N}angui {A}brogoua {U}niversity collection was used to confirm the varietal status in bottle gourd. {T}his analysis produced 22 575 single-nucleotide polymorphisms ({SNP}s). {C}luster analyses conducted with 2250 (9.96%) {SNP}s distinctly separated hard-shelled from soft-shelled types. {A}nalysis of 23 {SNP}s located in 11 genes coding for traits that differentiate the two types of gourds revealed that genes in the soft-shelled types had about 21% fewer {SNP}s than genes within hard-shelled gourds, but the latter had more non-synonymous {SNP}s. {C}luster analyses conducted with the 23 {SNP}s fitted well with the structure defined by the 2250 {SNP}s, suggesting the implication of these {SNP}s in the varietal differentiation of bottle gourd. {T}hese nucleotide changes along with the genetic relationships between the accessions provide molecular proof supporting the status of two varieties. {T}o prevent the confusion inherent in the use of synonyms and homonyms in bottle gourd, we suggest the terms hard-shelled and soft-shelled to designate, respectively, the varieties used as utensils and those grown for their edible seeds.},
  keywords = {{L}agenaria siceraria ; varietal identification ; genotyping-by-sequencing ; {SNP} ; {BURKINA} {FASO} ; {COTE} {D}'{IVOIRE} ; {MALI}},
  booktitle = {},
  journal = {{G}enome},
  volume = {63},
  numero = {11},
  pages = {535--545},
  ISSN = {0831-2796},
  year = {2020},
  DOI = {10.1139/gen-2020-0050},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079962},
}