Publications des scientifiques de l'IRD

Manasfi R., Chiron Serge, Montemurro N., Perez S., Brienza Monica. (2020). Biodegradation of fluoroquinolone antibiotics and the climbazole fungicide by Trichoderma species. Environmental Science and Pollution Research, 27 (18), 23331-23341. ISSN 0944-1344.

Titre du document
Biodegradation of fluoroquinolone antibiotics and the climbazole fungicide by Trichoderma species
Année de publication
2020
Type de document
Article référencé dans le Web of Science WOS:000528988900003
Auteurs
Manasfi R., Chiron Serge, Montemurro N., Perez S., Brienza Monica
Source
Environmental Science and Pollution Research, 2020, 27 (18), 23331-23341 ISSN 0944-1344
Filamentous fungi Trichoderma have been able to efficiently degrade fluoroquinolone antibiotics namely ciprofloxacin (CIP) and ofloxacin (OFL) as well as the fungicide climbazole (CLB) that are persistent in conventional activated sludge processes. All targeted compounds were biotransformed by whole cells of Trichoderma spp., exactly T. harzanium and T. asperellum, and biosorption played a limited role in their elimination. However, contrasting results were obtained with the two strains. T. asperellum was more efficient against CIP, with a 81% degradation rate in 13 days of incubation, while T. harzianum was more efficient against CLB, with a 91% degradation rate. While in the case of OFL, both strains showed same efficiency with degradation rate around 40%. Adding a cytochrome P450 enzyme inhibitor hardly resulted in the modification of degradation kinetics supporting the implication of extracellular enzymes in chemical biotransformation. Transformation products were identified by liquid chromatography-high resolution-mass spectrometry and transformation pathways were proposed. Biotransformation of selected compounds included hydroxylation, oxidation/reduction and N- and O-dealkylation reactions, similarly to those reported with white rot fungi. CIP underwent transformations at the piperazinyl ring through oxidation and conjugation reactions, while OFL mainly underwent hydroxylation processes and CLB carbonyl reduction into alcohol. Consequently, Trichoderma spp. likely possess a machinery of unspecific enzymes, which makes their application in removal of pharmaceutical and personal care products attractive.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Pollution [038] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010079031]
Identifiant IRD
fdi:010079031
Contact