Panpradist N., Beck I. A., Vrana J., Higa N., McIntyre D., Ruth P. S., So I., Kline E. C., Kanthula R., Wong-On-Wing A., Lim J., Ko D., Milne R., Rossouw T., Feucht U. D., Chung M., Jourdain Gonzague, Ngo-Giang-Huong Nicole, Laomanit L., Soria J., Lai J., Klavins E. D., Frenkel L. M., Lutz B. R. (2019). OLA-Simple : a software-guided HIV-1 drug resistance test for low-resource laboratories. Ebiomedicine, 50, p. 34-44. ISSN 2352-3964.
Titre du document
OLA-Simple : a software-guided HIV-1 drug resistance test for low-resource laboratories
Année de publication
2019
Auteurs
Panpradist N., Beck I. A., Vrana J., Higa N., McIntyre D., Ruth P. S., So I., Kline E. C., Kanthula R., Wong-On-Wing A., Lim J., Ko D., Milne R., Rossouw T., Feucht U. D., Chung M., Jourdain Gonzague, Ngo-Giang-Huong Nicole, Laomanit L., Soria J., Lai J., Klavins E. D., Frenkel L. M., Lutz B. R.
Source
Ebiomedicine, 2019,
50, p. 34-44 ISSN 2352-3964
Background: HIV drug resistance (HIVDR) testing can assist clinicians in selecting treatments. However, high complexity and cost of genotyping assays limit routine testing in settings where HIVDR prevalence has reached high levels. Methods: The oligonucleotide ligation assay (OLA)-Simple kit was developed for detection of HIVDR against first-line non-nucleoside/nucleoside reverse transcriptase inhibitors and validated on 672 codons (168 spedmens) from subtypes A, B, C, D, and AE. The kit uses dry reagents to facilitate assay setup, lateral flow devices for visual HIVDR detections, and in-house software with an interface for guiding users and analyzing results. Findings: HIVDR analysis of specimens by OLA-Simple compared to Sanger sequencing revealed 99.6 +/- 0.3% specificity and 98.2 +/- 0.9% sensitivity, and compared to high-sensitivity assays, 99.6 +/- 0.6% specificity and 86.2 +/- 2.5% sensitivity, with 2.6 +/- 0.9% indeterminate results. OLA-Simple was performed more rapidly compared to Sanger sequencing (<4 h vs. 35-72 h). Forty-one untrained volunteers blindly tested two specimens each with 96.8 +/- 0.8% accuracy. Interpretation: OLA-Simple compares favorably with HIVDR genotyping by Sanger and sensitive comparators. Instructional software enabled inexperienced, first-time users to perform the assay with high accuracy. The reduced complexity, cost, and training requirements of OLA-Simple could improve access to HIVDR testing in low-resource settings and potentially allow same-day selection of appropriate antiretroviral therapy.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
;
Informatique [122]
Localisation
Fonds IRD [F B010077728]
Identifiant IRD
fdi:010077728
