@article{fdi:010077728,
  title = {{OLA}-{S}imple : a software-guided {HIV}-1 drug resistance test for low-resource laboratories},
  author = {{P}anpradist, {N}. and {B}eck, {I}. {A}. and {V}rana, {J}. and {H}iga, {N}. and {M}c{I}ntyre, {D}. and {R}uth, {P}. {S}. and {S}o, {I}. and {K}line, {E}. {C}. and {K}anthula, {R}. and {W}ong-{O}n-{W}ing, {A}. and {L}im, {J}. and {K}o, {D}. and {M}ilne, {R}. and {R}ossouw, {T}. and {F}eucht, {U}. {D}. and {C}hung, {M}. and {J}ourdain, {G}onzague and {N}go-{G}iang-{H}uong, {N}icole and {L}aomanit, {L}. and {S}oria, {J}. and {L}ai, {J}. and {K}lavins, {E}. {D}. and {F}renkel, {L}. {M}. and {L}utz, {B}. {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {HIV} drug resistance ({HIVDR}) testing can assist clinicians in selecting treatments. {H}owever, high complexity and cost of genotyping assays limit routine testing in settings where {HIVDR} prevalence has reached high levels. {M}ethods: {T}he oligonucleotide ligation assay ({OLA})-{S}imple kit was developed for detection of {HIVDR} against first-line non-nucleoside/nucleoside reverse transcriptase inhibitors and validated on 672 codons (168 spedmens) from subtypes {A}, {B}, {C}, {D}, and {AE}. {T}he kit uses dry reagents to facilitate assay setup, lateral flow devices for visual {HIVDR} detections, and in-house software with an interface for guiding users and analyzing results. {F}indings: {HIVDR} analysis of specimens by {OLA}-{S}imple compared to {S}anger sequencing revealed 99.6 +/- 0.3% specificity and 98.2 +/- 0.9% sensitivity, and compared to high-sensitivity assays, 99.6 +/- 0.6% specificity and 86.2 +/- 2.5% sensitivity, with 2.6 +/- 0.9% indeterminate results. {OLA}-{S}imple was performed more rapidly compared to {S}anger sequencing (<4 h vs. 35-72 h). {F}orty-one untrained volunteers blindly tested two specimens each with 96.8 +/- 0.8% accuracy. {I}nterpretation: {OLA}-{S}imple compares favorably with {HIVDR} genotyping by {S}anger and sensitive comparators. {I}nstructional software enabled inexperienced, first-time users to perform the assay with high accuracy. {T}he reduced complexity, cost, and training requirements of {OLA}-{S}imple could improve access to {HIVDR} testing in low-resource settings and potentially allow same-day selection of appropriate antiretroviral therapy.},
  keywords = {{HIV} genotyping assay ; {NNRTI} ; {NRT} ; {R}esistance ; {R}egimen switching},
  booktitle = {},
  journal = {{E}biomedicine},
  volume = {50},
  numero = {},
  pages = {34--44},
  ISSN = {2352-3964},
  year = {2019},
  DOI = {10.1016/j.eblom.2019.11.002},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077728},
}