Bengue M., Ferraris Pauline, Baronti Cécile, Diagne C. T., Talignani L., Wichit S., Liégeois Florian, Bisbal C., Nougairede A., Missé Dorothée. (2019). Mayaro virus infects human chondrocytes and induces the expression of arthritis-related genes associated with joint degradation. Viruses, 11 (9), art. 797 [13 p.].
Titre du document
Mayaro virus infects human chondrocytes and induces the expression of arthritis-related genes associated with joint degradation
Année de publication
2019
Auteurs
Bengue M., Ferraris Pauline, Baronti Cécile, Diagne C. T., Talignani L., Wichit S., Liégeois Florian, Bisbal C., Nougairede A., Missé Dorothée
Source
Viruses, 2019,
11 (9), art. 797 [13 p.]
Mayaro virus (MAYV) is an emerging arthritogenic alphavirus belonging to the Togaviridae family. Infection leads to a dengue-like illness accompanied by severe polyarthralgia. However, the molecular and cellular mechanisms of arthritis as a result of MAYV infection remain poorly understood. In the present study, we assess the susceptibility of human chondrocytes (HC), fibroblast-like synoviocytes and osteoblasts that are the major cell types involved in osteoarthritis, to infection with MAYV. We show that these cells are highly permissive to MAYV infection and that viral RNA copy number and viral titers increase over time in infected cells. Knowing that HC are the primary cells in articular cartilage and are essential for maintaining the cartilaginous matrix, gene expression studies were conducted in MAYV-infected primary HC using polymerase chain reaction (PCR) arrays. The infection of the latter cells resulted in an induction in the expression of several matrix metalloproteinases (MMP) including MMP1, MMP7, MMP8, MMP10, MMP13, MMP14 and MMP15 which could be involved in the destruction of articular cartilage. Infected HC were also found to express significantly increased levels of various IFN-stimulated genes and arthritogenic mediators such as TNF-alpha and IL-6. In conclusion, MAYV-infected primary HC overexpress arthritis-related genes, which may contribute to joint degradation and pathogenesis.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010077079]
Identifiant IRD
fdi:010077079
