Publications des scientifiques de l'IRD

Souza Prado G., Bamogo P. K. A., Cordeiro de Abreu J. A., Gillet F. X., Olinto dos Santos V., Mattar Silva M. C., Brizard Jean-Paul, Porto Bemquerer M., Bangratz Martine, Brugidou Christophe, Sérémé D., Grossi-de-Sa M. F., Lacombe Séverine. (2019). Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil alpha-amylase. BMC Biotechnology, 19, p. art. 15 [13 p.]. ISSN 1472-6750.

Titre du document
Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil alpha-amylase
Année de publication
2019
Type de document
Article référencé dans le Web of Science WOS:000460741300001
Auteurs
Souza Prado G., Bamogo P. K. A., Cordeiro de Abreu J. A., Gillet F. X., Olinto dos Santos V., Mattar Silva M. C., Brizard Jean-Paul, Porto Bemquerer M., Bangratz Martine, Brugidou Christophe, Sérémé D., Grossi-de-Sa M. F., Lacombe Séverine
Source
BMC Biotechnology, 2019, 19, p. art. 15 [13 p.] ISSN 1472-6750
BackgroundInsect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolution strategies have been used on genes encoding these proteins to generate thousands of variants with new or improved functions. The cotton boll weevil (Anthonomus grandis) is one of the major pests of cotton in the Americas. An -amylase inhibitor (-AIC3) variant previously developed via molecular evolution strategy showed inhibitory activity against A. grandis -amylase (AGA).ResultsWe produced in a few days considerable amounts of -AIC3 using an optimised transient heterologous expression system in Nicotiana benthamiana. This high -AIC3 accumulation allowed its structural and functional characterizations. We demonstrated via MALDI-TOF MS/MS technique that the protein was processed as expected. It could inhibit up to 100% of AGA biological activity whereas it did not act on -amylase of two non-pathogenic insects. These data confirmed that N. benthamiana is a suitable and simple system for high-level production of biologically active -AIC3. Based on other benefits such as economic, health and environmental that need to be considerate, our data suggested that -AIC3 could be a very promising candidate for the production of transgenic crops resistant to cotton boll weevil without lethal effect on at least two non-pathogenic insects.ConclusionsWe propose this expression system can be complementary to molecular evolution strategies to identify the most promising variants before starting long-lasting stable transgenic programs.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Sciences du monde végétal [076] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010075306]
Identifiant IRD
fdi:010075306
Contact