@article{fdi:010075306,
  title = {{N}icotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil alpha-amylase},
  author = {{S}ouza {P}rado, {G}. and {B}amogo, {P}. {K}. {A}. and {C}ordeiro de {A}breu, {J}. {A}. and {G}illet, {F}. {X}. and {O}linto dos {S}antos, {V}. and {M}attar {S}ilva, {M}. {C}. and {B}rizard, {J}ean-{P}aul and {P}orto {B}emquerer, {M}. and {B}angratz, {M}artine and {B}rugidou, {C}hristophe and {S}{\'e}r{\'e}m{\'e}, {D}. and {G}rossi-de-{S}a, {M}. {F}. and {L}acombe, {S}{\'e}verine},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{I}nsect resistance in crops represents a main challenge for agriculture. {T}ransgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. {T}o extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolution strategies have been used on genes encoding these proteins to generate thousands of variants with new or improved functions. {T}he cotton boll weevil ({A}nthonomus grandis) is one of the major pests of cotton in the {A}mericas. {A}n -amylase inhibitor (-{AIC}3) variant previously developed via molecular evolution strategy showed inhibitory activity against {A}. grandis -amylase ({AGA}).{R}esults{W}e produced in a few days considerable amounts of -{AIC}3 using an optimised transient heterologous expression system in {N}icotiana benthamiana. {T}his high -{AIC}3 accumulation allowed its structural and functional characterizations. {W}e demonstrated via {MALDI}-{TOF} {MS}/{MS} technique that the protein was processed as expected. {I}t could inhibit up to 100% of {AGA} biological activity whereas it did not act on -amylase of two non-pathogenic insects. {T}hese data confirmed that {N}. benthamiana is a suitable and simple system for high-level production of biologically active -{AIC}3. {B}ased on other benefits such as economic, health and environmental that need to be considerate, our data suggested that -{AIC}3 could be a very promising candidate for the production of transgenic crops resistant to cotton boll weevil without lethal effect on at least two non-pathogenic insects.{C}onclusions{W}e propose this expression system can be complementary to molecular evolution strategies to identify the most promising variants before starting long-lasting stable transgenic programs.},
  keywords = {{T}ransient protein expression ; alpha-amylase inhibitors ; {G}ene silencing ; suppressors ; {C}otton boll weevil},
  booktitle = {},
  journal = {{BMC} {B}iotechnology},
  volume = {19},
  numero = {},
  pages = {art. 15 [13 p.]},
  ISSN = {1472-6750},
  year = {2019},
  DOI = {10.1186/s12896-019-0507-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075306},
}