Aubouy Agnès, Carme B. (2004). Plasmodium DNA contamination between blood smears during Giemsa staining and microscopic examination. Journal of Infectious Diseases, 190 (7), p. 1335-1337. ISSN 0022-1899.
Titre du document
Plasmodium DNA contamination between blood smears during Giemsa staining and microscopic examination
Journal of Infectious Diseases, 2004,
190 (7), p. 1335-1337 ISSN 0022-1899
Giemsa-stained blood smears are mainly used for microscopic examination to diagnose malaria. However, they may also be subjected to polymerase chain reaction (PCR) to confirm diagnosis or for retrospective studies requiring the analysis of old smears. We investigated the possibility of DNA contamination occurring during automated Giemsa staining or due to the failure to clean the oil-immersion objective during microscopic examination. We tested blood smears from uninfected, Plasmodium vivax-infected, and P. falciparum-infected patients. DNA contamination was observed after both staining and microscopy, although contamination was unpredictable during staining. These results are of utmost importance when smears are used for PCR.
Plan de classement
Epidémiologie du paludisme [052ANOPAL03]
Descripteurs
PALUDISME ; DIAGNOSTIC ; TRANSMISSION ; PCR.REACTION DE POLYMERISATION EN CHAINE
