@article{fdi:010073865,
  title = {{P}lasmodium {DNA} contamination between blood smears during {G}iemsa staining and microscopic examination},
  author = {{A}ubouy, {A}gn{\`e}s and {C}arme, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}iemsa-stained blood smears are mainly used for microscopic examination to diagnose malaria. {H}owever, they may also be subjected to polymerase chain reaction ({PCR}) to confirm diagnosis or for retrospective studies requiring the analysis of old smears. {W}e investigated the possibility of {DNA} contamination occurring during automated {G}iemsa staining or due to the failure to clean the oil-immersion objective during microscopic examination. {W}e tested blood smears from uninfected, {P}lasmodium vivax-infected, and {P}. falciparum-infected patients. {DNA} contamination was observed after both staining and microscopy, although contamination was unpredictable during staining. {T}hese results are of utmost importance when smears are used for {PCR}.},
  keywords = {{PALUDISME} ; {DIAGNOSTIC} ; {TRANSMISSION} ; {PCR}.{REACTION} {DE} {POLYMERISATION} {EN} {CHAINE}},
  booktitle = {},
  journal = {{J}ournal of {I}nfectious {D}iseases},
  volume = {190},
  numero = {7},
  pages = {1335--1337},
  ISSN = {0022-1899},
  year = {2004},
  DOI = {10.1086/424529},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073865},
}