Sekeyova Z., Mediannikov Oleg, Subramanian G., Kowalczewska M., Quevedo-Diaz M., Kocianova E., Rault D. (2012). Isolation of Rickettsia helvetica from ticks in Slovakia. Acta Virologica, 56 (3), p. 247-252. ISSN 0001-723X.
Titre du document
Isolation of Rickettsia helvetica from ticks in Slovakia
Sekeyova Z., Mediannikov Oleg, Subramanian G., Kowalczewska M., Quevedo-Diaz M., Kocianova E., Rault D.
Source
Acta Virologica, 2012,
56 (3), p. 247-252 ISSN 0001-723X
To date, only three rickettsial species have been found in ticks in Slovakia by serological and/or molecular-biological techniques, namely Rickettsia slovaca, Candidatus rickettsia IRS, and Rickettsia raoultii. Recently, we succeeded in isolation of the forth species, Rickettsia helvetica from Ixodes ricinus, the most frequent tick in Slovakia. The isolation, positive for 1096 of tested ticks, was performed on XTC cells by the shell-vial technique, Gimenez staining and light microscopy. The infected cell cultures contained rod-shaped particles morphologically identical to rickettsiae. The isolation was confirmed by direct detection of a fragment of the R. helvetica gene for citrate synthase in the positive ticks by PCR and its subsequent cloning, sequencing and comparison with the database.
