@article{fdi:010071503,
  title = {{I}solation of {R}ickettsia helvetica from ticks in {S}lovakia},
  author = {{S}ekeyova, {Z}. and {M}ediannikov, {O}leg and {S}ubramanian, {G}. and {K}owalczewska, {M}. and {Q}uevedo-{D}iaz, {M}. and {K}ocianova, {E}. and {R}ault, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o date, only three rickettsial species have been found in ticks in {S}lovakia by serological and/or molecular-biological techniques, namely {R}ickettsia slovaca, {C}andidatus rickettsia {IRS}, and {R}ickettsia raoultii. {R}ecently, we succeeded in isolation of the forth species, {R}ickettsia helvetica from {I}xodes ricinus, the most frequent tick in {S}lovakia. {T}he isolation, positive for 1096 of tested ticks, was performed on {XTC} cells by the shell-vial technique, {G}imenez staining and light microscopy. {T}he infected cell cultures contained rod-shaped particles morphologically identical to rickettsiae. {T}he isolation was confirmed by direct detection of a fragment of the {R}. helvetica gene for citrate synthase in the positive ticks by {PCR} and its subsequent cloning, sequencing and comparison with the database.},
  keywords = {{R}ickettsia helvetica ; isolation ; {I}xodes ricinus ; {S}lovakia ; {SLOVAQUE} {REPUBLIQUE}},
  booktitle = {},
  journal = {{A}cta {V}irologica},
  volume = {56},
  numero = {3},
  pages = {247--252},
  ISSN = {0001-723{X}},
  year = {2012},
  DOI = {10.4149/av_2012_03_247},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071503},
}