Publications des scientifiques de l'IRD

Atieh T., Baronti Cécile, Lamballerie Xavier de, Nougairède A. (2016). Simple reverse genetics systems for Asian and African Zika viruses. Scientific Reports - Nature, 6, art. 39384 [7 p.]. ISSN 2045-2322.

Titre du document
Simple reverse genetics systems for Asian and African Zika viruses
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000389877100001
Auteurs
Atieh T., Baronti Cécile, Lamballerie Xavier de, Nougairède A.
Source
Scientific Reports - Nature, 2016, 6, art. 39384 [7 p.] ISSN 2045-2322
Zika virus (ZIKV), a typical example of a re-emerging pathogen, recently caused large outbreaks in Pacific islands and the Americas, associated with congenital diseases and neurological complications. Deciphering the natural history, ecology and pathophysiology of this mosquito-borne pathogen requires effective reverse genetics tools. In the current study, using the bacterium-free 'Infectious Subgenomic Amplicons' (ISA) method, we generated and made available to the scientific community via the non-profit European Virus Archive collection, two simple and performing reverse genetics systems for ZIKV. One is based on an Asian ZIKV strain belonging to the outbreak lineage (French Polynesia 2013). The second was designed from the sequence of a low-passaged ZIKV African strain (Dakar 1984). Using the ISA procedure, we derived wild-type and a variety of specifically engineered ZIKVs in days (intra-and inter-lineage chimeras). Since they are based on low-passaged ZIKV strains, these engineered viruses provide ideal tools to study the effect of genetic changes observed in different evolutionary time-scales of ZIKV as well as pathophysiology of ZIKV infections.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
POLYNESIE FRANCAISE ; MARTINIQUE ; ASIE ; AFRIQUE
Localisation
Fonds IRD [F B010068774]
Identifiant IRD
fdi:010068774
Contact