@article{fdi:010068774,
  title = {{S}imple reverse genetics systems for {A}sian and {A}frican {Z}ika viruses},
  author = {{A}tieh, {T}. and {B}aronti, {C}{\'e}cile and {L}amballerie, {X}avier de and {N}ougair{\`e}de, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{Z}ika virus ({ZIKV}), a typical example of a re-emerging pathogen, recently caused large outbreaks in {P}acific islands and the {A}mericas, associated with congenital diseases and neurological complications. {D}eciphering the natural history, ecology and pathophysiology of this mosquito-borne pathogen requires effective reverse genetics tools. {I}n the current study, using the bacterium-free '{I}nfectious {S}ubgenomic {A}mplicons' ({ISA}) method, we generated and made available to the scientific community via the non-profit {E}uropean {V}irus {A}rchive collection, two simple and performing reverse genetics systems for {ZIKV}. {O}ne is based on an {A}sian {ZIKV} strain belonging to the outbreak lineage ({F}rench {P}olynesia 2013). {T}he second was designed from the sequence of a low-passaged {ZIKV} {A}frican strain ({D}akar 1984). {U}sing the {ISA} procedure, we derived wild-type and a variety of specifically engineered {ZIKV}s in days (intra-and inter-lineage chimeras). {S}ince they are based on low-passaged {ZIKV} strains, these engineered viruses provide ideal tools to study the effect of genetic changes observed in different evolutionary time-scales of {ZIKV} as well as pathophysiology of {ZIKV} infections.},
  keywords = {{POLYNESIE} {FRANCAISE} ; {MARTINIQUE} ; {ASIE} ; {AFRIQUE}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {6},
  numero = {},
  pages = {art. 39384 [7 ]},
  ISSN = {2045-2322},
  year = {2016},
  DOI = {10.1038/srep39384},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068774},
}