Publications des scientifiques de l'IRD

Nguyen T. D., Lacombe Séverine, Bangratz Martine, Ta H. A., Vinh D. N., Gantet Pascal, Brugidou Christophe. (2015). P2 of Rice grassy stunt virus (RGSV) and p6 and p9 of Rice ragged stunt virus (RRSV) isolates from Vietnam exert suppressor activity on the RNA silencing pathway. Virus Genes, 51 (2), p. 267-275. ISSN 0920-8569.

Titre du document
P2 of Rice grassy stunt virus (RGSV) and p6 and p9 of Rice ragged stunt virus (RRSV) isolates from Vietnam exert suppressor activity on the RNA silencing pathway
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000361459400012
Auteurs
Nguyen T. D., Lacombe Séverine, Bangratz Martine, Ta H. A., Vinh D. N., Gantet Pascal, Brugidou Christophe
Source
Virus Genes, 2015, 51 (2), p. 267-275 ISSN 0920-8569
In Vietnam, the two main viruses that cause disease in rice are the Rice grassy stunt virus (RGSV) and the Rice ragged stunt virus (RRSV). Outbreaks of these two viruses have dramatically decreased rice production in Vietnam. Because natural resistance genes are unknown, an RNAi strategy may be an alternative method to develop resistance to RGSV and RRSV. However, this strategy will be efficient only if putative silencing suppressors encoded by the two viruses are neutralized. To identify these suppressors, we used the classical green fluorescent protein (GFP) agroinfiltration method in Nicotiana benthamiana. Then, we investigated the effects of viral candidate proteins on GFP expression and GFP siRNA accumulation and their interference with the short- or long-range signal of silencing. RGSV genes s2gp1, s5gp2, and s6gp1 and RRSV genes s5gp1, s6gp1, s9gp1, and s10gp1 were selected for viral silencing suppressor investigation according to their small molecular weight, the presence of cysteines, or the presence of a GW motif in related protein products. We confirmed that protein p6 of RRSV displays mild silencing suppressor activity and affects long-range silencing by delaying the systemic silencing signal. In addition, we identified two new silencing suppressors that displayed mild activity: p2 of RGSV and p9 of RRSV.
Plan de classement
Sciences du monde végétal [076]
Description Géographique
VIET NAM
Localisation
Fonds IRD [F B010065308]
Identifiant IRD
fdi:010065308
Contact