Waithaisong K., Robin A., Martin A., Clairotte M., Villeneuve Manon, Plassard C. (2015). Quantification of organic P and low-molecular-weight organic acids in ferralsol soil extracts by ion chromatography. Geoderma, 257 (SI), p. 94-101. ISSN 0016-7061.
Titre du document
Quantification of organic P and low-molecular-weight organic acids in ferralsol soil extracts by ion chromatography
Waithaisong K., Robin A., Martin A., Clairotte M., Villeneuve Manon, Plassard C.
Source
Geoderma, 2015,
257 (SI), p. 94-101 ISSN 0016-7061
A better understanding of the composition of organic phosphorus (organic P) and low-molecular-weight organic acids (LMWOAs) in soil will make it possible to predict phosphorus cycling, improve biomass production and help phosphorus management in forest plantations. The method for quantifying organic P and LMWOAs in soil extracts was developed using ion chromatography (IC). Sodium hydroxide (0.5 N) was validated as an extractant for both organic P and LMWOAs. A mixture of 200 mM NaOH and ultrapure water was used to create a linear gradient elution. The eluent program separated five different organic P forms (glucose-6-phosphate, AMP, fructose-1,6-bisphosphate, ATP and phytate) as well as pyrophosphate from inorganic P (Pi), and four LMWOAs (malate, malonate, oxalate and citrate) in a 25 minute run through an AS11 column. The variability of the quantification was expressed as the relative standard deviation which was less than 20% for most of the analytes. The recovery rate of organic P assayed by IC to colorimetry was 110 +/- 20%. This chromatographic method was shown to be suitable for the quantification of organic P and LMWOAs in soil samples and could be an easier alternative to P-31 Nuclear Magnetic Resonance (NMR) measurement.
