@article{fdi:010064794,
  title = {{Q}uantification of organic {P} and low-molecular-weight organic acids in ferralsol soil extracts by ion chromatography},
  author = {{W}aithaisong, {K}. and {R}obin, {A}. and {M}artin, {A}. and {C}lairotte, {M}. and {V}illeneuve, {M}anon and {P}lassard, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} better understanding of the composition of organic phosphorus (organic {P}) and low-molecular-weight organic acids ({LMWOA}s) in soil will make it possible to predict phosphorus cycling, improve biomass production and help phosphorus management in forest plantations. {T}he method for quantifying organic {P} and {LMWOA}s in soil extracts was developed using ion chromatography ({IC}). {S}odium hydroxide (0.5 {N}) was validated as an extractant for both organic {P} and {LMWOA}s. {A} mixture of 200 m{M} {N}a{OH} and ultrapure water was used to create a linear gradient elution. {T}he eluent program separated five different organic {P} forms (glucose-6-phosphate, {AMP}, fructose-1,6-bisphosphate, {ATP} and phytate) as well as pyrophosphate from inorganic {P} ({P}i), and four {LMWOA}s (malate, malonate, oxalate and citrate) in a 25 minute run through an {AS}11 column. {T}he variability of the quantification was expressed as the relative standard deviation which was less than 20% for most of the analytes. {T}he recovery rate of organic {P} assayed by {IC} to colorimetry was 110 +/- 20%. {T}his chromatographic method was shown to be suitable for the quantification of organic {P} and {LMWOA}s in soil samples and could be an easier alternative to {P}-31 {N}uclear {M}agnetic {R}esonance ({NMR}) measurement.},
  keywords = {{O}rganic {P} forms ; {L}ow-molecular-weight organic acids ; {S}oil extract ; {S}ingle-run separation ; {IC} quantification ; {BRESIL}},
  booktitle = {},
  journal = {{G}eoderma},
  volume = {257},
  numero = {{SI}},
  pages = {94--101},
  ISSN = {0016-7061},
  year = {2015},
  DOI = {10.1016/j.geoderma.2014.12.024},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064794},
}