Hermand J.P., Randall J., Dubois F., Queeckers P., Yourassowsky C., Roubaud Fabrice, Grelet Jacques, Roudaut Gildas, Sarre A., Brehmer Patrice. (2013). In-situ holography microscopy of plankton and particles over the continental shelf of Senegal. In :
Pillai P.R.S. (ed.), Supriya M.H. (ed.). Proceedings of SYMPOL 2013. Piscataway : IEEE, p. 154-163. SYMPOL.Symposium on Ocean Electronics, 12., Kochi (IND), 2013/10/23-25. ISBN 9789380095455.
Titre du document
In-situ holography microscopy of plankton and particles over the continental shelf of Senegal
Année de publication
2013
Type de document
Partie d'ouvrage
Auteurs
Hermand J.P., Randall J., Dubois F., Queeckers P., Yourassowsky C., Roubaud Fabrice, Grelet Jacques, Roudaut Gildas, Sarre A., Brehmer Patrice
In
Pillai P.R.S. (ed.), Supriya M.H. (ed.), Proceedings of SYMPOL 2013
Source
Piscataway : IEEE, 2013,
p. 154-163 ISBN 9789380095455
Colloque
SYMPOL.Symposium on Ocean Electronics, 12., Kochi (IND), 2013/10/23-25
We present the first results obtained by a newly developed submersible digital holography microscope (DHM), Holoflow@Sea, to enable continuous in-situ monitoring of ocean or fresh water bodies in a less intrusive manner. The microscope features an off-axis configuration with reduced-coherence illumination. The optics is designed to image plankton and particles in the size range 2 ?m200 ?m within a water volume of 1 mm x 1 mm x 2 mm. The prototype was successfully deployed for the first time over the continental shelf of Senegal during a fisheries survey carried out in March 2013. The objective was to combine several laboratory techniques used for plankton and particle identification (highperformance liquid chromatography, flow cytometry and optical microscopy) on discrete collected samples with DHM images taken in situ at locations with different environmental conditions. Hologram data were acquired inside an upwelling cell, i.e., new water, and along the coast, i.e., old water, as well as off the upwelling cell at the continental shelf border. Preliminary results of holographic reconstruction are encouraging, with the distinctive morphology of some phytoplankton species allowing easy identification to genera level. Challenges are recognised with the identification of small spheroid organisms. Analyses are underway to allow comparison with traditional methods of plankton identification and evaluate the benefit of additional in-situ observations obtained by holography microscopy. The preliminary results already demonstrate the potential of DHM for in-situ studies of plankton and particles.
Plan de classement
Phytoplancton [034BIOVEG02]
;
Applications diverses [122APPLIC]
Descripteurs
MICROPLANCTON ; PARTICULE ; DIATOMEE ; IDENTIFICATION ; METHODE D'ANALYSE ; MICROSCOPIE ELECTRONIQUE ; IMAGE NUMERIQUE ; MESURE IN SITU ; UPWELLING
Description Géographique
SENEGAL ; ATLANTIQUE
Localisation
Fonds IRD [F B010064372]
Identifiant IRD
fdi:010064372
