Publications des scientifiques de l'IRD

Diouf F., Diouf D., Klonowska Agnieszka, Le Quéré Antoine, Bakhoum N., Fall D., Neyra Marc, Parrinello H., Diouf M., Ndoye I., Moulin Lionel. (2015). Genetic and genomic diversity studies of acacia symbionts in Senegal reveal new species of Mesorhizobium with a putative geographical pattern. Plos One, 10 (2), p. e0117667 [20 p.]. ISSN 1932-6203.

Titre du document
Genetic and genomic diversity studies of acacia symbionts in Senegal reveal new species of Mesorhizobium with a putative geographical pattern
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000349444900199
Auteurs
Diouf F., Diouf D., Klonowska Agnieszka, Le Quéré Antoine, Bakhoum N., Fall D., Neyra Marc, Parrinello H., Diouf M., Ndoye I., Moulin Lionel
Source
Plos One, 2015, 10 (2), p. e0117667 [20 p.] ISSN 1932-6203
Acacia senegal (L) Willd. and Acacia seyal Del. are highly nitrogen-fixing and moderately salt tolerant species. In this study we focused on the genetic and genomic diversity of Acacia mesorhizobia symbionts from diverse origins in Senegal and investigated possible correlations between the genetic diversity of the strains, their soil of origin, and their tolerance to salinity. We first performed a multi-locus sequence analysis on five markers gene fragments on a collection of 47 mesorhizobia strains of A. senegal and A. seyal from 8 localities. Most of the strains (60%) clustered with the M. plurifarium type strain ORS 1032(T), while the others form four new clades (MSP1 to MSP4). We sequenced and assembled seven draft genomes: four in the M. plurifarium clade (ORS3356, ORS3365, STM8773 and ORS1032T), one in MSP1 (STM8789), MSP2 (ORS3359) and MSP3 (ORS3324). The average nucleotide identities between these genomes together with the MLSA analysis reveal three new species of Mesorhizobium. A great variability of salt tolerance was found among the strains with a lack of correlation between the genetic diversity of mesorhizobia, their salt tolerance and the soils samples characteristics. A putative geographical pattern of A. senegal symbionts between the dryland north part and the center of Senegal was found, reflecting adaptations to specific local conditions such as the water regime. However, the presence of salt does not seem to be an important structuring factor of Mesorhizobiumspecies.
Plan de classement
Sciences du monde végétal [076] ; Biotechnologies [084]
Description Géographique
SENEGAL
Localisation
Fonds IRD [F B010063951]
Identifiant IRD
fdi:010063951
Contact