Mei N., Zergane N., Postec A., Erauso G., Oilier A., Payri Claude, Pelletier Bernard, Fardeau Marie-Laure, Ollivier Bernard, Quéméneur Marianne. (2014). Fermentative hydrogen production by a new alkaliphilic Clostridium sp (strain PROH2) isolated from a shallow submarine hydrothermal chimney in Prony Bay, New Caledonia. International Journal of Hydrogen Energy, 39 (34), p. 19465-19473. ISSN 0360-3199.
Titre du document
Fermentative hydrogen production by a new alkaliphilic Clostridium sp (strain PROH2) isolated from a shallow submarine hydrothermal chimney in Prony Bay, New Caledonia
Année de publication
2014
Auteurs
Mei N., Zergane N., Postec A., Erauso G., Oilier A., Payri Claude, Pelletier Bernard, Fardeau Marie-Laure, Ollivier Bernard, Quéméneur Marianne
Source
International Journal of Hydrogen Energy, 2014,
39 (34), p. 19465-19473 ISSN 0360-3199
The hydrogen-producing strain PROH2 pertaining to the genus Clostridium was successfully isolated from a shallow submarine hydrothermal chimney (Prony Bay, New Caledonia) driven by serpentinization processes. Cell biomass and hydrogen production performances during fermentation by strain PROH2 were studied in a series of batch experiments under various conditions of pH, temperature, NaCl and glucose concentrations. The highest hydrogen yield, 2.71 mol H-2/mol glucose, was observed at initial pH 9.5, 37 degrees C, and glucose concentration 2 g/L, and was comparable to that reported for neutrophilic clostridial species. Hydrogen production by strain PROH2 reached the maximum production rate (0.55 mM-H-2/h) at the late exponential phase. Yeast extract was required for growth of strain PROH2 and improved significantly its hydrogen production performances. The isolate could utilize various energy sources including cellobiose, galactose, glucose, Maltose, sucrose and trehalose to produce hydrogen. The pattern of end-products of metabolism was also affected by the type of energy sources and culture conditions used. These results indicate that Clostridium sp. strain PROH2 is a good candidate for producing hydrogen under alkaline and mesothermic conditions.
Plan de classement
Biotechnologies [084]
Description Géographique
NOUVELLE CALEDONIE
Localisation
Fonds IRD [F B010063518]
Identifiant IRD
fdi:010063518
