@article{fdi:010063518, title = {{F}ermentative hydrogen production by a new alkaliphilic {C}lostridium sp (strain {PROH}2) isolated from a shallow submarine hydrothermal chimney in {P}rony {B}ay, {N}ew {C}aledonia}, author = {{M}ei, {N}. and {Z}ergane, {N}. and {P}ostec, {A}. and {E}rauso, {G}. and {O}ilier, {A}. and {P}ayri, {C}laude and {P}elletier, {B}ernard and {F}ardeau, {M}arie-{L}aure and {O}llivier, {B}ernard and {Q}u{\'e}m{\'e}neur, {M}arianne}, editor = {}, language = {{ENG}}, abstract = {{T}he hydrogen-producing strain {PROH}2 pertaining to the genus {C}lostridium was successfully isolated from a shallow submarine hydrothermal chimney ({P}rony {B}ay, {N}ew {C}aledonia) driven by serpentinization processes. {C}ell biomass and hydrogen production performances during fermentation by strain {PROH}2 were studied in a series of batch experiments under various conditions of p{H}, temperature, {N}a{C}l and glucose concentrations. {T}he highest hydrogen yield, 2.71 mol {H}-2/mol glucose, was observed at initial p{H} 9.5, 37 degrees {C}, and glucose concentration 2 g/{L}, and was comparable to that reported for neutrophilic clostridial species. {H}ydrogen production by strain {PROH}2 reached the maximum production rate (0.55 m{M}-{H}-2/h) at the late exponential phase. {Y}east extract was required for growth of strain {PROH}2 and improved significantly its hydrogen production performances. {T}he isolate could utilize various energy sources including cellobiose, galactose, glucose, {M}altose, sucrose and trehalose to produce hydrogen. {T}he pattern of end-products of metabolism was also affected by the type of energy sources and culture conditions used. {T}hese results indicate that {C}lostridium sp. strain {PROH}2 is a good candidate for producing hydrogen under alkaline and mesothermic conditions.}, keywords = {{B}iohydrogen production ; {C}lostridium ; {H}ydrothermal system ; {A}lkaliphilic ; {S}erpentinization ; hyd{A} genes ; {NOUVELLE} {CALEDONIE}}, booktitle = {}, journal = {{I}nternational {J}ournal of {H}ydrogen {E}nergy}, volume = {39}, numero = {34}, pages = {19465--19473}, ISSN = {0360-3199}, year = {2014}, DOI = {10.1016/j.ijhydene.2014.09.111}, URL = {https://www.documentation.ird.fr/hor/fdi:010063518}, }