Publications des scientifiques de l'IRD

Hamad I., Keita M. B., Peeters Martine, Delaporte Eric, Raoult Didier, Bittar F. (2014). Pathogenic eukaryotes in gut microbiota of western lowland gorillas as revealed by molecular survey. Scientific Reports, 4, art. 6417 [9 p.]. ISSN 2045-2322.

Titre du document
Pathogenic eukaryotes in gut microbiota of western lowland gorillas as revealed by molecular survey
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000341942300006
Auteurs
Hamad I., Keita M. B., Peeters Martine, Delaporte Eric, Raoult Didier, Bittar F.
Source
Scientific Reports, 2014, 4, art. 6417 [9 p.] ISSN 2045-2322
Although gorillas regarded as the largest extant species of primates and have a close phylogenetic relationship with humans, eukaryotic communities have not been previously studied in these populations. Herein, 35 eukaryotic primer sets targeting the 18S rRNA gene, internal transcribed spacer gene and other specific genes were used firstly to explore the eukaryotes in a fecal sample from a wild western lowland gorilla (Gorilla gorilla gorilla). Then specific real-time PCRs were achieved in additional 48 fecal samples from 21 individual gorillas to investigate the presence of human eukaryotic pathogens. In total, 1,572 clones were obtained and sequenced from the 15 cloning libraries, resulting in the retrieval of 87 eukaryotic species, including 52 fungi, 10 protozoa, 4 nematodes and 21 plant species, of which 52, 5, 2 and 21 species, respectively, have never before been described in gorillas. We also reported the occurrence of pathogenic fungi and parasites (i.e. Oesophagostomum bifurcum (86%), Necator americanus (43%), Candida tropicalis (81%) and other pathogenic fungi were identified). In conclusion, molecular techniques using multiple primer sets may offer an effective tool to study complex eukaryotic communities and to identify potential pathogens in the gastrointestinal tracts of primates.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052] ; Sciences du monde animal [080]
Description Géographique
CAMEROUN ; AFRIQUE CENTRALE
Localisation
Fonds IRD [F B010062570]
Identifiant IRD
fdi:010062570
Contact