@article{fdi:010062570,
  title = {{P}athogenic eukaryotes in gut microbiota of western lowland gorillas as revealed by molecular survey},
  author = {{H}amad, {I}. and {K}eita, {M}. {B}. and {P}eeters, {M}artine and {D}elaporte, {E}ric and {R}aoult, {D}idier and {B}ittar, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}lthough gorillas regarded as the largest extant species of primates and have a close phylogenetic relationship with humans, eukaryotic communities have not been previously studied in these populations. {H}erein, 35 eukaryotic primer sets targeting the 18{S} r{RNA} gene, internal transcribed spacer gene and other specific genes were used firstly to explore the eukaryotes in a fecal sample from a wild western lowland gorilla ({G}orilla gorilla gorilla). {T}hen specific real-time {PCR}s were achieved in additional 48 fecal samples from 21 individual gorillas to investigate the presence of human eukaryotic pathogens. {I}n total, 1,572 clones were obtained and sequenced from the 15 cloning libraries, resulting in the retrieval of 87 eukaryotic species, including 52 fungi, 10 protozoa, 4 nematodes and 21 plant species, of which 52, 5, 2 and 21 species, respectively, have never before been described in gorillas. {W}e also reported the occurrence of pathogenic fungi and parasites (i.e. {O}esophagostomum bifurcum (86%), {N}ecator americanus (43%), {C}andida tropicalis (81%) and other pathogenic fungi were identified). {I}n conclusion, molecular techniques using multiple primer sets may offer an effective tool to study complex eukaryotic communities and to identify potential pathogens in the gastrointestinal tracts of primates.},
  keywords = {{CAMEROUN} ; {AFRIQUE} {CENTRALE}},
  booktitle = {},
  journal = {{S}cientific {R}eports},
  volume = {4},
  numero = {},
  pages = {art. 6417 [9 ]},
  ISSN = {2045-2322},
  year = {2014},
  DOI = {10.1038/srep06417},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062570},
}