Publications des scientifiques de l'IRD

Camara O., Camara M., Lejon Veerle, Ilboudo H., Sakande H., Leno M., Buscher P., Bucheton Bruno, Jamonneau Vincent. (2014). Immune trypanolysis test with blood spotted on filter paper for epidemiological surveillance of sleeping sickness. Tropical Medicine and International Health, 19 (7), p. 828-831. ISSN 1360-2276.

Titre du document
Immune trypanolysis test with blood spotted on filter paper for epidemiological surveillance of sleeping sickness
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000337796400009
Auteurs
Camara O., Camara M., Lejon Veerle, Ilboudo H., Sakande H., Leno M., Buscher P., Bucheton Bruno, Jamonneau Vincent
Source
Tropical Medicine and International Health, 2014, 19 (7), p. 828-831 ISSN 1360-2276
OBJECTIVES The immune trypanolysis test (TL) is an accurate sero-diagnostic tool increasingly implemented for sleeping sickness medical surveillance, but it is restricted to the reference laboratories. To facilitate storage and transport of the test specimen, we developed a protocol for the examination of blood spotted on filter paper (TL-fp) that can be stored and shipped at ambient temperature. We compared its performance with the classical TL on plasma (TL-pl) that needs to be kept frozen until use. METHODS The study was conducted in active foci of the Republic of Guinea. In total, 438 specimens from treated and untreated sleeping sickness patients and serological suspects were tested with both methods. RESULT TL-fp gave significantly less positive results than TL-pl, but all the confirmed sleeping sickness cases were positive with the TL-fp protocol. CONCLUSION TL-fp appears to offer a good compromise between feasibility and sensitivity to detect currently infected subjects who play a role in the transmission of Trypanosoma brucei gambiense and is useful for contributing to the elimination of gambiense sleeping sickness.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
GUINEE
Localisation
Fonds IRD [F B010062305]
Identifiant IRD
fdi:010062305
Contact