Horizon / Plein textes La base de ressources documentaires de l'IRD



Publications des scientifiques de l'IRD

Lemaire J., Mkannez G., Guerfali F. Z., Gustin C., Attia H., Sghaier R. M., Dellagi Koussay, Laouini D., Renard P. (2013). MicroRNA expression profile in human macrophages in response to Leishmania major infection. Plos Neglected Tropical Diseases, 7 (10), e2478. ISSN 1935-2735

Fichier PDF disponiblehttp://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010061738.pdf[ PDF Link ]

Lien direct chez l'éditeur doi:10.1371/journal.pntd.0002478

En Libre Accès sur HAL http://hal.univ-reunion.fr/hal-01285440

MicroRNA expression profile in human macrophages in response to Leishmania major infection
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000330376500018
AuteursLemaire J., Mkannez G., Guerfali F. Z., Gustin C., Attia H., Sghaier R. M., Dellagi Koussay, Laouini D., Renard P.
SourcePlos Neglected Tropical Diseases, 2013, 7 (10), p. e2478. p. e2478 ISSN 1935-2735
RésuméBackground: Leishmania (L.) are intracellular protozoan parasites able to survive and replicate in the hostile phagolysosomal environment of infected macrophages. They cause leishmaniasis, a heterogeneous group of worldwide-distributed affections, representing a paradigm of neglected diseases that are mainly embedded in impoverished populations. To establish successful infection and ensure their own survival, Leishmania have developed sophisticated strategies to subvert the host macrophage responses. Despite a wealth of gained crucial information, these strategies still remain poorly understood. MicroRNAs (miRNAs), an evolutionarily conserved class of endogenous 22-nucleotide non-coding RNAs, are described to participate in the regulation of almost every cellular process investigated so far. They regulate the expression of target genes both at the levels of mRNA stability and translation; changes in their expression have a profound effect on their target transcripts. Methodology/Principal Findings: We report in this study a comprehensive analysis of miRNA expression profiles in L. major-infected human primary macrophages of three healthy donors assessed at different time-points post-infection (three to 24 h). We show that expression of 64 out of 365 analyzed miRNAs was consistently deregulated upon infection with the same trends in all donors. Among these, several are known to be induced by TLR-dependent responses. GO enrichment analysis of experimentally validated miRNA-targeted genes revealed that several pathways and molecular functions were disturbed upon parasite infection. Finally, following parasite infection, miR-210 abundance was enhanced in HIF-1 alpha-dependent manner, though it did not contribute to inhibiting anti-apoptotic pathways through pro-apoptotic caspase-3 regulation. Conclusions/Significance: Our data suggest that alteration in miRNA levels likely plays an important role in regulating macrophage functions following L. major infection. These results could contribute to better understanding of the dynamics of gene expression in host cells during leishmaniasis.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052]
LocalisationFonds IRD [F B010061738]
Identifiant IRDfdi:010061738
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010061738

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL

* PDF Link :

    à télécharger pour citer/partager ce document sur les réseaux sociaux académiques

Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation


Montpellier (centre IRD)

Montpellier (MSE)









La Paz