@article{fdi:010061738,
  title = {{M}icro{RNA} expression profile in human macrophages in response to {L}eishmania major infection},
  author = {{L}emaire, {J}. and {M}kannez, {G}. and {G}uerfali, {F}. {Z}. and {G}ustin, {C}. and {A}ttia, {H}. and {S}ghaier, {R}. {M}. and {D}ellagi, {K}oussay and {L}aouini, {D}. and {R}enard, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {L}eishmania ({L}.) are intracellular protozoan parasites able to survive and replicate in the hostile phagolysosomal environment of infected macrophages. {T}hey cause leishmaniasis, a heterogeneous group of worldwide-distributed affections, representing a paradigm of neglected diseases that are mainly embedded in impoverished populations. {T}o establish successful infection and ensure their own survival, {L}eishmania have developed sophisticated strategies to subvert the host macrophage responses. {D}espite a wealth of gained crucial information, these strategies still remain poorly understood. {M}icro{RNA}s (mi{RNA}s), an evolutionarily conserved class of endogenous 22-nucleotide non-coding {RNA}s, are described to participate in the regulation of almost every cellular process investigated so far. {T}hey regulate the expression of target genes both at the levels of m{RNA} stability and translation; changes in their expression have a profound effect on their target transcripts. {M}ethodology/{P}rincipal {F}indings: {W}e report in this study a comprehensive analysis of mi{RNA} expression profiles in {L}. major-infected human primary macrophages of three healthy donors assessed at different time-points post-infection (three to 24 h). {W}e show that expression of 64 out of 365 analyzed mi{RNA}s was consistently deregulated upon infection with the same trends in all donors. {A}mong these, several are known to be induced by {TLR}-dependent responses. {GO} enrichment analysis of experimentally validated mi{RNA}-targeted genes revealed that several pathways and molecular functions were disturbed upon parasite infection. {F}inally, following parasite infection, mi{R}-210 abundance was enhanced in {HIF}-1 alpha-dependent manner, though it did not contribute to inhibiting anti-apoptotic pathways through pro-apoptotic caspase-3 regulation. {C}onclusions/{S}ignificance: {O}ur data suggest that alteration in mi{RNA} levels likely plays an important role in regulating macrophage functions following {L}. major infection. {T}hese results could contribute to better understanding of the dynamics of gene expression in host cells during leishmaniasis.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {7},
  numero = {10},
  pages = {e2478},
  ISSN = {1935-2735},
  year = {2013},
  DOI = {10.1371/journal.pntd.0002478},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061738},
}