Publications des scientifiques de l'IRD

Zhao Shuai, Mo W. L., Wu F., Tang W., Tang J. L., Szurek Boris, Verdier Valérie, Koebnik Ralf, Feng J. X. (2013). Identification of non-TAL effectors in Xanthomonas oryzae pv. oryzae Chinese strain 13751 and analysis of their role in the bacterial virulence. World Journal of Microbiology and Biotechnology, 29 (4), p. 733-744. ISSN 0959-3993.

Titre du document
Identification of non-TAL effectors in Xanthomonas oryzae pv. oryzae Chinese strain 13751 and analysis of their role in the bacterial virulence
Année de publication
2013
Type de document
Article référencé dans le Web of Science WOS:000316291000017
Auteurs
Zhao Shuai, Mo W. L., Wu F., Tang W., Tang J. L., Szurek Boris, Verdier Valérie, Koebnik Ralf, Feng J. X.
Source
World Journal of Microbiology and Biotechnology, 2013, 29 (4), p. 733-744 ISSN 0959-3993
Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice bacterial leaf blight, one of the most important rice bacterial diseases in China and many other countries. The upstream portions of 41 candidate genes encoding non-transcription activator-like effectors of Xoo Chinese strain 13751 were fused with the coding sequence of AvrBs1(59-445) in a broad host-range vector. The constructed plasmids were respectively introduced into Xoo strain 13751 and avrBs1 deletion mutant of X. campestris pv. campestris strain 8004 by tri-parental conjugation. The resultant transconjugants were respectively tested for hypersensitive response (HR) elicitation on pepper ECW-10R. Nine strains were able to elicit HR on pepper, indicating that the nine genes (XOO0037, XOO0103, XOO0110, XOO0315, XOO1488, XOO2875, XOO3150, XOO3222 and XOO4134) encoded effectors. Among them, xopAE (13751) (XOO0110), expressed in Xoo strain 13751 growing in rice leaves, was a new experimentally confirmed effector gene. XopAE(13751) contains 11 leucine rich repeats. Furthermore, mutants for the nine effector genes were created in Xoo strain 13751 and subsequently tested for virulence in rice. As a result, only the xopR (13751) (XOO4134) deletion mutant GXMxopR showed a significant reduction in virulence in hybrid rice cv. Teyou63 compared to the wild type. However, the growth of GXMxopR in host plant rice was not affected. These results indicated that xopR (13751) was required for full virulence of Xoo strain 13751 by inducing rice disease tolerance.
Plan de classement
Sciences du monde végétal [076] ; Biotechnologies [084]
Description Géographique
CHINE
Localisation
Fonds IRD [F B010060753]
Identifiant IRD
fdi:010060753
Contact