Publications des scientifiques de l'IRD

Bodnar A. M., Bernal A., Szurek Boris, Lopez C. E. (2013). Tell me a tale of TALEs. Molecular Biotechnology, 53 (2), p. 228-235. ISSN 1073-6085.

Titre du document
Tell me a tale of TALEs
Année de publication
2013
Type de document
Article référencé dans le Web of Science WOS:000312723600013
Auteurs
Bodnar A. M., Bernal A., Szurek Boris, Lopez C. E.
Source
Molecular Biotechnology, 2013, 53 (2), p. 228-235 ISSN 1073-6085
Pathogenic bacteria of the Xanthomonas and Ralstonia genus have developed resourceful strategies creating a favorable environment to multiply and colonize their host plants. One of these strategies involves the secretion and translocation of several families of effector proteins into the host cell. The transcription activator-like effector (TALE) family forms a subset of proteins involved in the direct modulation of host gene expression. TALEs include a number of tandem 34-amino acid repeats in their central part, where specific residues variable in two adjacent positions determine DNA-binding in the host genome. The specificity of this binding and its predictable nature make TALEs a revolutionary tool for gene editing, functional analysis, modification of target gene expression, and directed mutagenesis. Several examples have been reported in higher organisms as diverse as plants, Drosophila, zebrafish, mouse, and even human cells. Here, we summarize the functions of TALEs in their natural context and the biotechnological perspectives of their use.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
Localisation
Fonds IRD [F B010058243]
Identifiant IRD
fdi:010058243
Contact