Godreuil Sylvain, Torrea G., Terru D., Chevenet François, Diagbouga S., Supply P., Van de Perre P., Carrière C., Banuls Anne-Laure. (2007). First molecular epidemiology study of Mycobacterium tuberculosis in Burkina Faso. Journal of Clinical Microbiology, 45 (3), p. 921-927. ISSN 0095-1137.
Titre du document
First molecular epidemiology study of Mycobacterium tuberculosis in Burkina Faso
Année de publication
2007
Auteurs
Godreuil Sylvain, Torrea G., Terru D., Chevenet François, Diagbouga S., Supply P., Van de Perre P., Carrière C., Banuls Anne-Laure
Source
Journal of Clinical Microbiology, 2007,
45 (3), p. 921-927 ISSN 0095-1137
We conducted a molecular epidemiology study on 120 Mycobacterium tuberculosis isolates from patients presenting pulmonary tuberculosis (TB) in Burkina Faso. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and spolligotyping, were applied after culture. Molecular analysis of specific signatures showed that all TB cases reported in this study were caused by M. tuberculosis and identified no Mycobacterium bovis or Mycobacterium africanum isolates. This result is unexpected, as M. africanum strains were reportedly the etiologic agent in 20% of TB cases 2 decades ago. The comparison of spoligotypes from Burkina Faso with an international spoligotype database (Spo1DB4) showed that the majority of isolates belong to major clades of M. tuberculosis (Haarlem, 9%; Latin American-Mediterranean, 30%; and T, 20%). The predominant group of isolates (30%) corresponds to spoligotype 61, described in Cameroon as the "Cameroon family." In Burkina Faso, as in Cameroon, this family could be associated with recent transmission of TB, suggesting a recent expansion in West Africa. Our data suggest a low level of primary drug resistance that may be a positive result of the Directly Observed Therapy Shortcourse program. Besides, based on spoligotyping plus MIRU-VNTR, data showed a high number of clusters in our sample, suggesting a high level of recent TB transmission in Burkina Faso. Nevertheless, an important genetic polymorphism was observed in this country, reflecting an endemicity situation where the control of TB would have less impact in the main towns.
Plan de classement
Santé : généralités [050]
Localisation
Fonds IRD [F B010057515]
Identifiant IRD
fdi:010057515
