Exploring proteins in Anopheles gambiae male and female antennae through MALDI mass spectrometry profiling

2008

Article

Dani F.R., Francese S., Mastrobuoni g., Felicioli A., Caputo B., Simard Frédéric, Pieraccini G., Moneti G., Coluzzi M., Della Torre A., Turillazzi S.

PloS One, 2008, 3 (7), e2822

MALDI profiling and imaging mass spectrometry (IMS) are novel techniques for direct analysis of peptides and smallproteins in biological tissues. In this work we applied them to the study ofAnopheles gambiaeantennae, with the aim ofanalysing expression of soluble proteins involved in olfaction perireceptor events. MALDI spectra obtained by directprofiling on single antennae and by the analysis of extracts, showed similar profiles, although spectra obtained throughprofiling had a richer ion population and higher signal to noise ratio. Male and female antennae showed distinct proteinprofiles. MALDI imaging experiments were also performed and differences were observed in the localization of someproteins. Two proteins were identified through high resolution measurement and top-down MS/MS experiments. A 8 kDa protein only present in the male antennae matched with an unannotated sequence of theAn. gambiaegenome, while thepresence of odorant binding protein 9 (OBP-9) was confirmed through experiments of 2-DE, followed by MS and MS/MSanalysis of digested spots. This work shows that MALDI MS profiling is a technique suitable for the analysis of proteins ofsmall and medium MW in insect appendices, and allows obtaining data for several specimens which can be investigated fordifferences between groups. Proteins of interest can be identified through other complementary MS approaches.

Anophèles et paludisme [052ANOPAL]

PALUDISME ; VECTEUR ; MOUSTIQUE ; MALE ; FEMELLE ; PREFERENCE TROPHIQUE ; PROTEINE ; SPECTROMETRIE ; PERCEPTION SENSORIELLE ; CONTACT HOMME VECTEUR ; CAPACITE VECTORIELLE

CAMEROUN

Fonds IRD [F B010054703]

fdi:010054703