@article{fdi:010054703,
  title = {{E}xploring proteins in {A}nopheles gambiae male and female antennae through {MALDI} mass spectrometry profiling},
  author = {{D}ani, {F}.{R}. and {F}rancese, {S}. and {M}astrobuoni, g. and {F}elicioli, {A}. and {C}aputo, {B}. and {S}imard, {F}r{\'e}d{\'e}ric and {P}ieraccini, {G}. and {M}oneti, {G}. and {C}oluzzi, {M}. and {D}ella {T}orre, {A}. and {T}urillazzi, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{MALDI} profiling and imaging mass spectrometry ({IMS}) are novel techniques for direct analysis of peptides and smallproteins in biological tissues. {I}n this work we applied them to the study of{A}nopheles gambiaeantennae, with the aim ofanalysing expression of soluble proteins involved in olfaction perireceptor events. {MALDI} spectra obtained by directprofiling on single antennae and by the analysis of extracts, showed similar profiles, although spectra obtained throughprofiling had a richer ion population and higher signal to noise ratio. {M}ale and female antennae showed distinct proteinprofiles. {MALDI} imaging experiments were also performed and differences were observed in the localization of someproteins. {T}wo proteins were identified through high resolution measurement and top-down {MS}/{MS} experiments. {A} 8 k{D}a protein only present in the male antennae matched with an unannotated sequence of the{A}n. gambiaegenome, while thepresence of odorant binding protein 9 ({OBP}-9) was confirmed through experiments of 2-{DE}, followed by {MS} and {MS}/{MS}analysis of digested spots. {T}his work shows that {MALDI} {MS} profiling is a technique suitable for the analysis of proteins ofsmall and medium {MW} in insect appendices, and allows obtaining data for several specimens which can be investigated fordifferences between groups. {P}roteins of interest can be identified through other complementary {MS} approaches.},
  keywords = {{PALUDISME} ; {VECTEUR} ; {MOUSTIQUE} ; {MALE} ; {FEMELLE} ; {PREFERENCE} {TROPHIQUE} ; {PROTEINE} ; {SPECTROMETRIE} ; {PERCEPTION} {SENSORIELLE} ; {CONTACT} {HOMME} {VECTEUR} ; {CAPACITE} {VECTORIELLE} ; {CAMEROUN}},
  booktitle = {},
  journal = {{P}lo{S} {O}ne},
  volume = {3},
  numero = {7},
  pages = {e2822},
  year = {2008},
  DOI = {10.1371/journal.pone.0002822},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054703},
}