Ruiz C., Haddad Mohamed, Alban J., Bourdy Geneviève, Reategui R., Castillo D., Sauvain Michel, Deharo Eric, Estevez Yannick, Arevalo J., Rojas R. (2011). Activity-guided isolation of antileishmanial compounds from Piper hispidum. Phytochemistry Letters, 4 (3), p. 363-366. ISSN 1874-3900.
Titre du document
Activity-guided isolation of antileishmanial compounds from Piper hispidum
Année de publication
2011
Auteurs
Ruiz C., Haddad Mohamed, Alban J., Bourdy Geneviève, Reategui R., Castillo D., Sauvain Michel, Deharo Eric, Estevez Yannick, Arevalo J., Rojas R.
Source
Phytochemistry Letters, 2011,
4 (3), p. 363-366 ISSN 1874-3900
The bioassay-guided purification of the ethanolic extract from the leaves of Piper hispidum led to the isolation of one new amide, N-2-(3',4',5'-trimethoxyphenyl)ethyl-2-hydroxybenzamide (1) as well as two known chalcones 2'-hydroxy-3',4',6'-trimethoxychalcone (2); 2',4'-dihydroxy-6'-methoxychalcone (cardamonin, 3) and one known flavanone, 5,7-dihydroxyflavanone (Pinocembrin, 4). Their structures were elucidated on the basis of spectroscopic data, including homo-and heteronuclear correlation NMR experiments (COSY, HSQC and HMBC) and comparison with data reported in the literature. The isolated compounds were tested against Leishmania amazonensis axenic amastigotes. The results showed that the known chalcone 2 exhibited the most potent antileishmanial activity with an IC50 of 0.8 mu M (amphotericin B: IC50 = 0.2 mu M) but was shown to exhibit mild cytotoxicity.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
;
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010054162]
Identifiant IRD
fdi:010054162
