Sajini K. K., Karun A., Amarnath C. H., Engelmann Florent. (2011). Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos by vitrification. Cryoletters, 32 (4), p. 317-328. ISSN 0143-2044.
Titre du document
Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos by vitrification
Sajini K. K., Karun A., Amarnath C. H., Engelmann Florent
Source
Cryoletters, 2011,
32 (4), p. 317-328 ISSN 0143-2044
The present study investigates the effect of preculture conditions, vitrification and unloading solutions on survival and regeneration of coconut zygotic embryos after cryopreservation. Among the seven plant vitrification solutions tested, PVS3 was found to be the most effective for regeneration of cryopreserved embryos. The optimal protocol involved preculture of embryos for 3 days on medium with 0.6 M sucrose, PVS3 treatment for 16 h, rapid cooling and rewarming and unloading in 1.2 M sucrose liquid medium for 1.5 h. Under these conditions, 70-80% survival (corresponding to size enlargement and weight gain) was observed with cryopreserved embryos and 20-25% of the plants regenerated (showing normal shoot and root growth) from cryopreserved embryos were established in pots.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
