@article{fdi:010053867,
  title = {{C}ryopreservation of coconut ({C}ocos nucifera {L}.) zygotic embryos by vitrification},
  author = {{S}ajini, {K}. {K}. and {K}arun, {A}. and {A}marnath, {C}. {H}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he present study investigates the effect of preculture conditions, vitrification and unloading solutions on survival and regeneration of coconut zygotic embryos after cryopreservation. {A}mong the seven plant vitrification solutions tested, {PVS}3 was found to be the most effective for regeneration of cryopreserved embryos. {T}he optimal protocol involved preculture of embryos for 3 days on medium with 0.6 {M} sucrose, {PVS}3 treatment for 16 h, rapid cooling and rewarming and unloading in 1.2 {M} sucrose liquid medium for 1.5 h. {U}nder these conditions, 70-80% survival (corresponding to size enlargement and weight gain) was observed with cryopreserved embryos and 20-25% of the plants regenerated (showing normal shoot and root growth) from cryopreserved embryos were established in pots.},
  keywords = {{C}ocos nucifera {L}. ; coconut ; zygotic embryo ; preculture ; vitrification ; {PVS}3 ; unloading},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {32},
  numero = {4},
  pages = {317--328},
  ISSN = {0143-2044},
  year = {2011},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053867},
}