Publications des scientifiques de l'IRD

Yu Yanhua, Streubel J., Balzergue S., Champion Antony, Boch J., Koebnik Ralf, Feng J. X., Verdier Valérie, Szurek Boris. (2011). Colonization of rice leaf blades by an African strain of Xanthomonas oryzae pv. oryzae depends on a new TAL effector that Induces the rice nodulin-3 os11N3 gene. Molecular Plant-Microbe Interactions, 24 (9), p. 1102-1113. ISSN 0894-0282.

Titre du document
Colonization of rice leaf blades by an African strain of Xanthomonas oryzae pv. oryzae depends on a new TAL effector that Induces the rice nodulin-3 os11N3 gene
Année de publication
2011
Type de document
Article référencé dans le Web of Science WOS:000293878600011
Auteurs
Yu Yanhua, Streubel J., Balzergue S., Champion Antony, Boch J., Koebnik Ralf, Feng J. X., Verdier Valérie, Szurek Boris
Source
Molecular Plant-Microbe Interactions, 2011, 24 (9), p. 1102-1113 ISSN 0894-0282
African strains of Xanthomonas oryzae pv. oryzae contain fewer TAL effectors than Asian strains, and their contribution to pathogenicity is unknown. Systematic mutagenesis of tal genes was used to decipher the contribution of each of the eight TAL effector paralogs to pathogenicity of African X. oryzae pv. oryzae BAI3. A strain mutated in talC was severely affected in the production of disease symptoms. Analysis of growth in planta upon leaf-clip inoculation showed that mutant bacteria multiplied only at the site of inoculation at the apex of the leaf, suggesting a requirement for talC during colonization of vascular tissues. Such tissue-specific effect of a tal mutant is a novel phenotype, which has not yet been characterized in other xanthomonads. Microarray experiments comparing the host response of rice leaves challenged with BAI3(R) vs. BAI3(R)Delta talC were performed to identify genes targeted by TalC. A total of 120 upregulated and 21 downregulated genes were identified, among them Os11N3, which is a member of the MtN3/saliva family. Based on semiquantitative reverse transcription-polymerase chain reaction and beta-glucuronidase reporter assays, we show that Os11N3 is directly upregulated by TalC and identify a TalC DNA target box within the Os11N3 upstream sequence.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010053778]
Identifiant IRD
fdi:010053778
Contact