Yu Yanhua auteur aut IRD Streubel J. auteur aut IRD Balzergue S. auteur aut IRD Champion Antony auteur aut IRD Boch J. auteur aut IRD Koebnik Ralf auteur aut IRD Feng J. X. auteur aut IRD Verdier Valérie auteur aut IRD Szurek Boris auteur aut IRD text journalArticle eng text/pdf born digital access African strains of Xanthomonas oryzae pv. oryzae contain fewer TAL effectors than Asian strains, and their contribution to pathogenicity is unknown. Systematic mutagenesis of tal genes was used to decipher the contribution of each of the eight TAL effector paralogs to pathogenicity of African X. oryzae pv. oryzae BAI3. A strain mutated in talC was severely affected in the production of disease symptoms. Analysis of growth in planta upon leaf-clip inoculation showed that mutant bacteria multiplied only at the site of inoculation at the apex of the leaf, suggesting a requirement for talC during colonization of vascular tissues. Such tissue-specific effect of a tal mutant is a novel phenotype, which has not yet been characterized in other xanthomonads. Microarray experiments comparing the host response of rice leaves challenged with BAI3(R) vs. BAI3(R)Delta talC were performed to identify genes targeted by TalC. A total of 120 upregulated and 21 downregulated genes were identified, among them Os11N3, which is a member of the MtN3/saliva family. Based on semiquantitative reverse transcription-polymerase chain reaction and beta-glucuronidase reporter assays, we show that Os11N3 is directly upregulated by TalC and identify a TalC DNA target box within the Os11N3 upstream sequence. specialized 076 24 9 1102-1113 2011 0894-0282 https://www.documentation.ird.fr/hor/fdi:010053778 10.1094/mpmi-11-10-0254 0894-0282 [F B010053778] https://www.documentation.ird.fr/hor/fdi:010053778 https://www.documentation.ird.fr/intranet/publi/2011/09/010053778.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-10-06 2017-08-23 fdi:010053778 fre