Diagne Nathalie, Escoute J., Lartaud M., Verdeil J. L., Franche Claudine, Kane A., Bogusz Didier, Diouf D., Duponnois Robin, Svistoonoff Sergio. (2011). Uvitex2B : a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots. Mycorrhiza, 21 (4), p. 315-321. ISSN 0940-6360.
Titre du document
Uvitex2B : a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots
Diagne Nathalie, Escoute J., Lartaud M., Verdeil J. L., Franche Claudine, Kane A., Bogusz Didier, Diouf D., Duponnois Robin, Svistoonoff Sergio
Source
Mycorrhiza, 2011,
21 (4), p. 315-321 ISSN 0940-6360
The study of arbuscular mycorrhiza often requires the staining of fungal structures using specific dyes. Fluorescent dyes such as acid fuchsin and wheat germ agglutinin conjugates give excellent results, but these compounds are either hazardous or very expensive. Here, we show that a safer and inexpensive dye, Uvitex2B, can be efficiently used to stain intraradical fungal structures formed by the arbuscular mycorrhizal fungus Glomus intraradices in three plant species: carrot, Casuarina equisetifolia, and Medicago truncatula. The intensity and stability of Uvitex2B allow the acquisition of high-quality images using not only confocal laser scanning microscopy but also epifluorescence microscopy coupled with image deconvolution. Furthermore, we demonstrate that Uvitex2B and beta-glucuronidase staining are compatible and can thus be used to reveal arbuscular mycorrhizal structures in the context of promoter activation analysis.
