@article{fdi:010053494,
  title = {{U}vitex2{B} : a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots},
  author = {{D}iagne, {N}athalie and {E}scoute, {J}. and {L}artaud, {M}. and {V}erdeil, {J}. {L}. and {F}ranche, {C}laudine and {K}ane, {A}. and {B}ogusz, {D}idier and {D}iouf, {D}. and {D}uponnois, {R}obin and {S}vistoonoff, {S}ergio},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he study of arbuscular mycorrhiza often requires the staining of fungal structures using specific dyes. {F}luorescent dyes such as acid fuchsin and wheat germ agglutinin conjugates give excellent results, but these compounds are either hazardous or very expensive. {H}ere, we show that a safer and inexpensive dye, {U}vitex2{B}, can be efficiently used to stain intraradical fungal structures formed by the arbuscular mycorrhizal fungus {G}lomus intraradices in three plant species: carrot, {C}asuarina equisetifolia, and {M}edicago truncatula. {T}he intensity and stability of {U}vitex2{B} allow the acquisition of high-quality images using not only confocal laser scanning microscopy but also epifluorescence microscopy coupled with image deconvolution. {F}urthermore, we demonstrate that {U}vitex2{B} and beta-glucuronidase staining are compatible and can thus be used to reveal arbuscular mycorrhizal structures in the context of promoter activation analysis.},
  keywords = {{A}rbuscular mycorrhizal fungi ({AMF}) ; {U}vitex2{B} ; {C}onfocal laser scanning ; microscopy ({CLSM}) ; {P}ro{M}t{E}nod11::gus},
  booktitle = {},
  journal = {{M}ycorrhiza},
  volume = {21},
  numero = {4},
  pages = {315--321},
  ISSN = {0940-6360},
  year = {2011},
  DOI = {10.1007/s00572-010-0357-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053494},
}