Cavel E., Pillot Marion, Pontier D., Lahmy S., Bies-Etheve N., Vega D., Grimanelli Daniel, Lagrange T. (2011). A plant-specific transcription factor IIB-related protein, pBRP2, is involved in endosperm growth control. Plos One, 6 (2), p. e17216. ISSN 1932-6203.
Titre du document
A plant-specific transcription factor IIB-related protein, pBRP2, is involved in endosperm growth control
Année de publication
2011
Auteurs
Cavel E., Pillot Marion, Pontier D., Lahmy S., Bies-Etheve N., Vega D., Grimanelli Daniel, Lagrange T.
Source
Plos One, 2011,
6 (2), p. e17216 ISSN 1932-6203
General transcription factor IIB (TFIIB) and TFIIB-related factor (BRF), are conserved RNA polymerase II/III (RNAPII/III) selectivity factors that are involved in polymerase recruitment and transcription initiation in eukaryotes. Recent findings have shown that plants have evolved a third type of B-factor, plant-specific TFIIB-related protein 1 (pBRP1), which seems to be involved in RNAPI transcription. Here, we extend the repertoire of B-factors in plants by reporting the characterization of a novel TFIIB-related protein, plant-specific TFIIB-related protein 2 (pBRP2), which is found to date only in the Brassicacea family. Unlike other B-factors that are ubiquitously expressed, PBRP2 expression is restricted to reproductive organs and seeds as shown by RT-PCR, immunofluorescence labelling and GUS staining experiments. Interestingly, pbrp2 loss-of-function specifically affects the development of the syncytial endosperm, with both parental contributions required for wild-type development. pBRP2, is the first B-factor to exhibit cell-specific expression and regulation in eukaryotes, and might play a role in enforcing bi-parental reproduction in angiosperms.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010053365]
Identifiant IRD
fdi:010053365
