%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cavel, E.
%A Pillot, Marion
%A Pontier, D.
%A Lahmy, S.
%A Bies-Etheve, N.
%A Vega, D.
%A Grimanelli, Daniel
%A Lagrange, T.
%T A plant-specific transcription factor IIB-related protein, pBRP2, is involved in endosperm growth control
%D 2011
%L fdi:010053365
%G ENG
%J Plos One
%@ 1932-6203
%M ISI:000287761700036
%N 2
%P e17216
%R 10.1371/journal.pone.0017216
%U https://www.documentation.ird.fr/hor/fdi:010053365
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-08/010053365.pdf
%V 6
%W Horizon (IRD)
%X General transcription factor IIB (TFIIB) and TFIIB-related factor (BRF), are conserved RNA polymerase II/III (RNAPII/III) selectivity factors that are involved in polymerase recruitment and transcription initiation in eukaryotes. Recent findings have shown that plants have evolved a third type of B-factor, plant-specific TFIIB-related protein 1 (pBRP1), which seems to be involved in RNAPI transcription. Here, we extend the repertoire of B-factors in plants by reporting the characterization of a novel TFIIB-related protein, plant-specific TFIIB-related protein 2 (pBRP2), which is found to date only in the Brassicacea family. Unlike other B-factors that are ubiquitously expressed, PBRP2 expression is restricted to reproductive organs and seeds as shown by RT-PCR, immunofluorescence labelling and GUS staining experiments. Interestingly, pbrp2 loss-of-function specifically affects the development of the syncytial endosperm, with both parental contributions required for wild-type development. pBRP2, is the first B-factor to exhibit cell-specific expression and regulation in eukaryotes, and might play a role in enforcing bi-parental reproduction in angiosperms.
%$ 076