Roopesh K., Guyot S., Sabu A., Haridas M., Perraud Gaime Isabelle, Roussos Sevastianos, Augur Christopher. (2010). Biotransformation of procyanidins by a purified fungal dioxygenase : identification and characterization of the products using mass spectrometry. Process Biochemistry, 45 (6), p. 904-913. ISSN 1359-5113.
Titre du document
Biotransformation of procyanidins by a purified fungal dioxygenase : identification and characterization of the products using mass spectrometry
Roopesh K., Guyot S., Sabu A., Haridas M., Perraud Gaime Isabelle, Roussos Sevastianos, Augur Christopher
Source
Process Biochemistry, 2010,
45 (6), p. 904-913 ISSN 1359-5113
Procyanidins commonly known as condensed tannins are a type of polyphenol with wide abundance naturally. They are commonly known as potent anti-oxidants with powerful free radical scavenging activity as well as anti-tumor-promoting activity. Little is known about the enzymatic mechanisms/pathways involved in the microbial biotransformation of these polyphenolic molecules. The extracellular enzyme, dioxygenase produced by Aspergillus fumigatus was used as in vitro tools to study the degradation pathway of a model procyanidin dimer, namely procyanidin B2. The enzyme was purified to homogeneity by a two step process of anion-exchange chromatography coupled with FPLC followed by gel-filtration chromatography coupled with HPLC and the molecular mass estimated. In addition, the different biotransformed products resulted from the dioxygenase action on P82 were purified using Reversed-Phase-High Performance Liquid Chromatography prior to their identification and characterization by structural elucidation using Electrospray Ionization-Mass Spectrometry. Subsequently, the mechanism of dioxygenase action on procyanidin dimer was defined.
