Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Process Biochemistry 904-913 Procyanidins Aspergillus fumigatus Dioxygenase Purification Reversed-Phase High Performance Liquid Chromatography Electrospray Ionization-Mass Spectrometry 2010 fdi:010049513 ENG Process Biochemistry 1359-5113 ISI:000277908100013 6 10.1016/j.procbio.2010.02.019 https://www.documentation.ird.fr/hor/fdi:010049513 https://www.documentation.ird.fr/intranet/publi/2010/06-1/010049513.pdf 45 Horizon (IRD) Procyanidins commonly known as condensed tannins are a type of polyphenol with wide abundance naturally. They are commonly known as potent anti-oxidants with powerful free radical scavenging activity as well as anti-tumor-promoting activity. Little is known about the enzymatic mechanisms/pathways involved in the microbial biotransformation of these polyphenolic molecules. The extracellular enzyme, dioxygenase produced by Aspergillus fumigatus was used as in vitro tools to study the degradation pathway of a model procyanidin dimer, namely procyanidin B2. The enzyme was purified to homogeneity by a two step process of anion-exchange chromatography coupled with FPLC followed by gel-filtration chromatography coupled with HPLC and the molecular mass estimated. In addition, the different biotransformed products resulted from the dioxygenase action on P82 were purified using Reversed-Phase-High Performance Liquid Chromatography prior to their identification and characterization by structural elucidation using Electrospray Ionization-Mass Spectrometry. Subsequently, the mechanism of dioxygenase action on procyanidin dimer was defined. 084 UR193 Inde