Bittar F., Ouchenane Z., Smati F., Raoult Didier, Rolain J. M. (2009). MALDI-TOF-MS for rapid detection of staphylococcal Panton-Valentine leukocidin. International Journal of Antimicrobial Agents, 34 (5), p. 467-470. ISSN 0924-8579.
Titre du document
MALDI-TOF-MS for rapid detection of staphylococcal Panton-Valentine leukocidin
Bittar F., Ouchenane Z., Smati F., Raoult Didier, Rolain J. M.
Source
International Journal of Antimicrobial Agents, 2009,
34 (5), p. 467-470 ISSN 0924-8579
Toxin-producing Gram-positive bacteria are responsible for emerging and life-threatening infections in humans worldwide. Both rapid toxin detection and adapted therapy are essential to limit the morbidity due to such toxins, especially staphylococcal Panton-Valentine leukocidin (PVL). Here we describe the use of a mass spectrometry profile generated by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) followed by ClinProTools (TM) 2.0 software analysis to find a reproducible model able to identify PVL in Staphylococcus aureus strains. Eighty-one S. aureus strains were used and tested for the presence of PVL, toxic shock syndrome toxin (TSST-1) and mecA genes. The peak at 4448 mass-to-charge ratio (m/z) was the most relevant peak to differentiate between PVL-producing and non-PVL-producing S. aureus. A model using only this peak had an overall recognition capability of 100% and an overall cross-validation of 77.07%. Prospective evaluation of the model allowed two cases of PVL-producing strains to be detected within a few minutes during the time of care and before polymerase chain reaction (PCR) results. Our study represents a proof of concept for the use of such rapid technology as a point-of-care method to identify potential lethal toxin quickly. We believe that such a rapid method will be timely to help change the therapeutic strategy and could be used in the future for other pathogens and infectious diseases.
