%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Bittar, F.
%A Ouchenane, Z.
%A Smati, F.
%A Raoult, Didier
%A Rolain, J. M.
%T MALDI-TOF-MS for rapid detection of staphylococcal Panton-Valentine leukocidin
%D 2009
%L fdi:010048268
%G ENG
%J International Journal of Antimicrobial Agents
%@ 0924-8579
%K Staphylococcus aureus ; Panton-Valentine leukocidin ; Mass spectrometry ; Toxin production ; Virulence factor
%M ISI:000270544200015
%N 5
%P 467-470
%R 10.1016/j.ijantimicag.2009.03.017
%U https://www.documentation.ird.fr/hor/fdi:010048268
%> https://www.documentation.ird.fr/intranet/publi/2009/10/010048268.pdf
%V 34
%W Horizon (IRD)
%X Toxin-producing Gram-positive bacteria are responsible for emerging and life-threatening infections in humans worldwide. Both rapid toxin detection and adapted therapy are essential to limit the morbidity due to such toxins, especially staphylococcal Panton-Valentine leukocidin (PVL). Here we describe the use of a mass spectrometry profile generated by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) followed by ClinProTools (TM) 2.0 software analysis to find a reproducible model able to identify PVL in Staphylococcus aureus strains. Eighty-one S. aureus strains were used and tested for the presence of PVL, toxic shock syndrome toxin (TSST-1) and mecA genes. The peak at 4448 mass-to-charge ratio (m/z) was the most relevant peak to differentiate between PVL-producing and non-PVL-producing S. aureus. A model using only this peak had an overall recognition capability of 100% and an overall cross-validation of 77.07%. Prospective evaluation of the model allowed two cases of PVL-producing strains to be detected within a few minutes during the time of care and before polymerase chain reaction (PCR) results. Our study represents a proof of concept for the use of such rapid technology as a point-of-care method to identify potential lethal toxin quickly. We believe that such a rapid method will be timely to help change the therapeutic strategy and could be used in the future for other pathogens and infectious diseases.
%$ 052