Simon Gwénola, Walther J., Zabeti Nathalie, Combet-Blanc Yannick, Auria Richard, van der Oost J., Casalot Laurence
Source
Fems Microbiology Letters, 2009,
299 (2), p. 255-260 ISSN 0378-1097
Sulfolobus solfataricus P2 was grown aerobically at various O-2 concentrations. Based on growth parameters in microcosms, four types of behavior could be distinguished. At 35% O-2 (v/v; gas phase), the cultures did not grow, indicating a lethal dose of oxygen. For 26-32% O-2, the growth was significantly affected compared with the reference (21%), suggesting a moderate toxicity by O-2. For 16-24% O-2, standard growth was observed. For 1.5-15% O-2, growth was comparable with the reference, but the yield on O-2 indicated a more efficient use of oxygen. These results indicate that S. solfataricus P2 grows optimally in the range of 1.5-24% O-2, most likely by adjusting its energy-transducing machinery. To gain some insight into control of the respiratory system, transcriptomes of the strain cultivated at different O-2 concentrations, corresponding to each behavior (1.5%, 21% and 26%), were compared using a DNA microarray approach. It showed differential expression of several genes encoding terminal oxidases, indicating an adaptation of the strain's respiratory system in response to fluctuating oxygen concentrations.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Biotechnologies [084]
