%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Simon, Gwénola
%A Walther, J.
%A Zabeti, Nathalie
%A Combet-Blanc, Yannick
%A Auria, Richard
%A van der Oost, J.
%A Casalot, Laurence
%T Effect of O-2 concentrations on Sulfolobus solfataricus P2
%D 2009
%L fdi:010048227
%G ENG
%J Fems Microbiology Letters
%@ 0378-1097
%K archaea ; Sulfolobus solfataricus ; oxygen ; physiology ; regulation of ; transcription
%M ISI:000269866500018
%N 2
%P 255-260
%R 10.1111/j.1574-6968.2009.01759.x
%U https://www.documentation.ird.fr/hor/fdi:010048227
%> https://www.documentation.ird.fr/intranet/publi/2009/10/010048227.pdf
%V 299
%W Horizon (IRD)
%X Sulfolobus solfataricus P2 was grown aerobically at various O-2 concentrations. Based on growth parameters in microcosms, four types of behavior could be distinguished. At 35% O-2 (v/v; gas phase), the cultures did not grow, indicating a lethal dose of oxygen. For 26-32% O-2, the growth was significantly affected compared with the reference (21%), suggesting a moderate toxicity by O-2. For 16-24% O-2, standard growth was observed. For 1.5-15% O-2, growth was comparable with the reference, but the yield on O-2 indicated a more efficient use of oxygen. These results indicate that S. solfataricus P2 grows optimally in the range of 1.5-24% O-2, most likely by adjusting its energy-transducing machinery. To gain some insight into control of the respiratory system, transcriptomes of the strain cultivated at different O-2 concentrations, corresponding to each behavior (1.5%, 21% and 26%), were compared using a DNA microarray approach. It showed differential expression of several genes encoding terminal oxidases, indicating an adaptation of the strain's respiratory system in response to fluctuating oxygen concentrations.
%$ 020 ; 084