Publications des scientifiques de l'IRD

Casasola A., Ramos-Cerrillo B., de Roodt A. R., Saucedo A. C., Chippaux Jean-Philippe, Alagon A., Stock R. P. (2009). Paraspecific neutralization of the venom of African species of cobra by an equine antiserum against Naja melanoleuca : a comparative study. Toxicon, 53 (6), p. 602-608. ISSN 0041-0101.

Titre du document
Paraspecific neutralization of the venom of African species of cobra by an equine antiserum against Naja melanoleuca : a comparative study
Année de publication
2009
Type de document
Article référencé dans le Web of Science WOS:000265358600002
Auteurs
Casasola A., Ramos-Cerrillo B., de Roodt A. R., Saucedo A. C., Chippaux Jean-Philippe, Alagon A., Stock R. P.
Source
Toxicon, 2009, 53 (6), p. 602-608 ISSN 0041-0101
Venoms of snakes belonging to the same Genera tend to share biochemical, toxinological and antigenic characteristics. Accordingly, paraspecific neutralization of venom lethality by experimental antisera and commercial antivenoms has been reported. We studied the spectrum of neutralization of lethality of an experimental monovalent equine antiserum against the strongly neurotoxic African forest cobra (Naja melanoleuca) when tested against venoms of most species of African Naja, both neuro and cytotoxic as described by some authors. We report a comparison of the median lethal doses (LD50) of the venoms and the paraspecific median effective doses (ED50) of the antiserum calculated using three methods: Spearman-Karber and Probit (currently recommended by the World Health Organization), and non-linear regression. An ample - but not complete - spectrum of paraspecific neutralization of lethality was observed against both spitting and non-spitting species of African Naja with a clearly more efficient neutralization of the more potent venoms, the implications of which are discussed. The median lethal and effective doses calculated by the three methods are remarkably consistent and may warrant consideration of non-linear regression methods for the calculation of venom lethality and antivenom potency by venom/antivenom researchers and producers.
Plan de classement
Santé : généralités [050]
Localisation
Fonds IRD [F B010046049]
Identifiant IRD
fdi:010046049
Contact