Gauthier Nathalie, Dalleau-Clouet C., Bouvret M. E. (2008). Twelve new polymorphic microsatellite loci and PCR multiplexing in the whitefly, Bemisia tabaci. Molecular Ecology Resources, 8 (5), p. 1004-1007. ISSN 1755-098X.
Titre du document
Twelve new polymorphic microsatellite loci and PCR multiplexing in the whitefly, Bemisia tabaci
Gauthier Nathalie, Dalleau-Clouet C., Bouvret M. E.
Source
Molecular Ecology Resources, 2008,
8 (5), p. 1004-1007 ISSN 1755-098X
Twelve new dinucleotide microsatellite loci of the whitefly, Bemisia tabaci, were obtained from enriched genomic libraries. Three polymerase chain reaction multiplex sets comprising three, five and four loci were optimized and characterized across 133 B. tabaci females from Israeli rearings and natural populations collected in four Mediterranean countries (Tunisia, France, Spain and Morocco). There were three to 24 alleles per locus and the observed heterozygosity was from 0.084 to 0.420. Deviation from Hardy-Weinberg equilibrium was detected at four loci associated with significant heterozygote deficiencies due to null alleles and presence of subpopulations that were mostly in the Tunisian sample. The 12 loci carried independent information.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
