Gauthier Nathalie auteur aut IRD Dalleau-Clouet C. auteur aut IRD Bouvret M. E. auteur aut IRD text journalArticle eng text/pdf born digital access Twelve new dinucleotide microsatellite loci of the whitefly, Bemisia tabaci, were obtained from enriched genomic libraries. Three polymerase chain reaction multiplex sets comprising three, five and four loci were optimized and characterized across 133 B. tabaci females from Israeli rearings and natural populations collected in four Mediterranean countries (Tunisia, France, Spain and Morocco). There were three to 24 alleles per locus and the observed heterozygosity was from 0.084 to 0.420. Deviation from Hardy-Weinberg equilibrium was detected at four loci associated with significant heterozygote deficiencies due to null alleles and presence of subpopulations that were mostly in the Tunisian sample. The 12 loci carried independent information. specialized B- and q-biotypes Bemisia tabaci Invasive pest Microsatellites Multiplex pcr Aleyrodidae Hemiptera Biotypes 076 020 8 5 1004-1007 2008 1755-098X https://www.documentation.ird.fr/hor/fdi:010042775 10.1111/j.1755-0998.2008.02163.x 1755-098X [F B010042775] https://www.documentation.ird.fr/hor/fdi:010042775 https://www.documentation.ird.fr/intranet/publi/2008/10/010042775.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-11-07 2024-06-21 fdi:010042775 fre